Back to Search
Start Over
Cellular immune responses in amniotic fluid of women with preterm clinical chorioamnionitis.
- Source :
-
Inflammation Research . Feb2020, Vol. 69 Issue 2, p203-216. 14p. - Publication Year :
- 2020
-
Abstract
- Objective: Preterm birth is the leading cause of neonatal morbidity and mortality worldwide. Some preterm births are associated with clinical chorioamnionitis; yet, this condition has been poorly investigated. Herein, we characterized the amniotic fluid cellular immune responses in women with preterm clinical chorioamnionitis. Methods and subjects: Amniotic fluid samples were obtained from women with preterm clinical chorioamnionitis and a positive or negative microbiological culture (n = 17). The cellular composition of amniotic fluid was evaluated using fluorescence microscopy, scanning and transmission electron microscopy, and flow cytometry. Women without preterm clinical chorioamnionitis were also examined (n = 10). Results: Amniotic fluid from women with preterm clinical chorioamnionitis and a positive culture had: (1) abundant neutrophils associated with viable and non-viable bacteria, (2) neutrophils performing phagocytosis, (3) neutrophils forming NETs, (4) increased numbers of neutrophils, monocytes/macrophages, and CD4+ T cells, and (5) high expression of IL-1β by neutrophils and monocytes/macrophages. Amniotic fluid from women with preterm clinical chorioamnionitis and proven infection tended to have fewer monocytes/macrophages and CD4+ T cells compared to those without chorioamnionitis. Conclusion: We provide the first morphologic and phenotypic characterization of the cellular immune responses in the amniotic cavity of women with preterm clinical chorioamnionitis, a condition associated with adverse neonatal outcomes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10233830
- Volume :
- 69
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Inflammation Research
- Publication Type :
- Academic Journal
- Accession number :
- 141453551
- Full Text :
- https://doi.org/10.1007/s00011-019-01308-x