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A characterization of HULIS-C and the oxidative potential of HULIS and HULIS-Fe(II) mixture in PM2.5 during hazy and non-hazy days in Shanghai.
- Source :
-
Atmospheric Environment . Dec2019, Vol. 219, pN.PAG-N.PAG. 1p. - Publication Year :
- 2019
-
Abstract
- Humic-like substances (HULIS) were isolated from PM 2.5 in Shanghai. The oxidative potential and the cytotoxicity of the isolated HULIS and its Fe(II) binary mixtures were evaluated by using cell-free Dithiothreitol (DTT) and cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Our data showed that HULIS-C contents in spring during hazy and non-hazy days were relatively higher than in winter. Cell-free DTT and bioassay (reactive oxygen species (ROS) and MTT) results showed that higher ROS values were released from individual HULIS or SRFA (Suwannee River Fulvic Acid) compounds than HULIS-Fe(II) complexes and Fe(II) alone. In addition, DTT and ROS correlation results were in line with each other and the oxidative potentials of HULIS and its binary mixtures were strongly inter-correlated with carbonaceous components (WSOC, OC) in PM 2.5. Dose-dependent MTT results revealed that cell mortality positively correlated with the mass concentrations of HULIS (10–50 μg/ml). In the HULIS-Fe(II) system, the ROS generation ability depended on the mixing time of HULIS-C with Fe(II), thus suggesting that the organic-metal complexation time and the Fenton reaction induced by Fe (II) play a role in the cell mortality rate. Image 1 • Higher reactive oxygen species from HULIS-Fe(II) complexation than Fe (II) alone. • Reactive oxygen species generation depending on the mixing time with Fe(II). • Oxidative potentials of HULIS and its binary mixtures strongly inter-correlated with WSOC/ OC. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13522310
- Volume :
- 219
- Database :
- Academic Search Index
- Journal :
- Atmospheric Environment
- Publication Type :
- Academic Journal
- Accession number :
- 141579871
- Full Text :
- https://doi.org/10.1016/j.atmosenv.2019.117058