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Maternal regulation of SATB2 in osteo‐progeniters impairs skeletal development in offspring.
- Source :
-
FASEB Journal . Feb2020, Vol. 34 Issue 2, p2511-2523. 13p. - Publication Year :
- 2020
-
Abstract
- Nutritional status during intrauterine and/or early postnatal life has substantial influence on adult offspring health. Along these lines, there is a growing body of evidence illustrating that high fat diet (HFD)‐induced maternal obesity can regulate fetal bone development. Thus, we investigated the effects of maternal obesity on both fetal skeletal development and mechanisms linking maternal obesity to osteoblast differentiation in offspring. Embryonic osteogenic calvarial cells (EOCCs) were isolated from fetuses at gestational day 18.5 (E18.5) of HFD‐induced obese rat dams. We observed impaired differentiation of EOCCs to mature osteoblasts from HFD obese dams. ChIP‐seq‐based genome‐wide localization of the repressive histone mark H3K27me3 (mediated via the polycomb histone methyltransferase, enhancer of zeste homologue 2 [Ezh2]) showed that this phenotype was associated with increased enrichment of H3K27me3 on the gene of SATB2, a critical transcription factor required for osteoblast differentiation. Knockdown of Ezh2 in EOCCs and ST2 cells increased SATB2 expression; while Ezh2 overexpression in EOCCs and ST2 cells decreased SATB2 expression. These data were consistent with experimental results showing strong association between H3K27me3, Ezh2, and SATB2 in cells from rats and humans. We have further presented that SATB2 mRNA and protein expression were increased in bones, and increased trabecular bone mass from pre‐osteoblast specific Ezh2 deletion (Ezh2flox/flox Osx‐Cre+ cko) mice compared with those from control Cre+ mice. These findings indicate that maternal HFD‐induced obesity may be associated with decreasing fetal pre‐osteoblastic cell differentiation, under epigenetic control of SATB2 expression via Ezh2‐dependent mechanisms. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08926638
- Volume :
- 34
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- FASEB Journal
- Publication Type :
- Academic Journal
- Accession number :
- 141676162
- Full Text :
- https://doi.org/10.1096/fj.201901901R