Molecular identification of Culicoides (Diptera: Ceratopogonidae) species in Algeria.

• Bluetongue virus is transmitted by Culicoides species. • Molecular diagnosis was conducted in Culicoides midges collected in Algeria. • The presence of 14 species were identified in Algeria. • Geographic distribution and potential role of bluetongue virus transmission was discussed in identified species. Bluetongue is a serious vector-borne viral disease that infects wild and domestic ruminants. The causative virus is transmitted by midges of the genus Culicoides , which consists of at least 1350 species worldwide. Since 1998, bluetongue disease has spread to Europe and northern Africa, including Algeria. To better understand the distribution of Culicoides species in Algeria, adult midges were collected from 17 different regions in Algeria from 2009 to 2015. At first, 492 specimens were grouped into 52 batches by wing patterns and geographic area of Algeria. Analysis of 60 nucleotide sequences of the mitochondrial cytochrome oxidase subunit I (COI) gene showed that the presence of 14 species including five unknown species, which were belonged to seven distinct subgenera. At least five species (C. imicola, C. obsoletus, C. puncticollis, C. kingi , and C. newsteadi) were discussed as potential vectors of bluetongue virus (BTV). The present study provides important insights into the genetic diversity of Culicoides and the potential spread of BTV in Algeria. Image, graphical abstract [ABSTRACT FROM AUTHOR]