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Parameritannin A-2 from Urceola huaitingii enhances doxorubicin-induced mitochondria-dependent apoptosis by inhibiting the PI3K/Akt, ERK1/2 and p38 pathways in gastric cancer cells.
- Source :
-
Chemico-Biological Interactions . Jan2020, Vol. 316, pN.PAG-N.PAG. 1p. - Publication Year :
- 2020
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Abstract
- Parameritannin A-2 (PA-2) is a natural product extracted from the stems of the plant Urceola huaitingii. Our previous studies have shown that PA-2 exhibits significant synergistic anticancer effects with doxorubicin (DOX) in HGC27 gastric cancer cell lines. Here we report that our isobolographic analysis confirms the synergistic cytotoxic effects of PA-2 and DOX in HGC27 cells. Flow cytometry and immunoblotting indicate that PA-2 enhances DOX-mediated apoptosis. Importantly, PA-2 enhances the intracellular accumulation of DOX in HGC27 cells. The combination of DOX and PA-2 remarkably increases the release of cytochrome C and the activation of caspase-3 and caspase-9, compared with DOX treatment alone. Moreover, PA-2 attenuates the DOX-induced activation of Akt, ERK1/2 and p38 signaling pathways, providing a molecular mechanism for the synergistic effects of DOX and PA-2 in the induction of apoptosis. In conclusion, our studies demonstrate that PA-2 and DOX synergistically induce mitochondria-dependent apoptosis as PA-2 inhibits the PI3K/Akt, ERK1/2 and p38 pathways in HGC27 cells. These findings suggest that the combination treatment with PA-2 and DOX may represent a potent therapy for gastric cancer. • PA-2 exhibits significant synergistic anticancer effects with DOX in HGC27 cells. • PA-2 enhances the intracellular accumulation of DOX in HGC27 cells. • PA-2 enhances DOX-mediated apoptosis. • PA-2 attenuates the DOX-induced activation of Akt, ERK1/2 and p38 signaling pathways. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00092797
- Volume :
- 316
- Database :
- Academic Search Index
- Journal :
- Chemico-Biological Interactions
- Publication Type :
- Academic Journal
- Accession number :
- 141808272
- Full Text :
- https://doi.org/10.1016/j.cbi.2019.108924