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Single-excited double-emission CdTe@CdS quantum dots for use in a fluorometric hybridization assay for multiple tumor-related microRNAs.
- Source :
-
Microchimica Acta . Feb2020, Vol. 187 Issue 2, p1-9. 9p. - Publication Year :
- 2020
-
Abstract
- A method is described for the simultaneous determination of hepatocellular carcinoma-associated microRNA-122 and microRNA-199a/b-3p. This probe consists of two kinds of nanomaterials. The first comprises CdTe@CdS core-shell quantum dots which, on excitation at 375 nm give two emissions, with peak wavelengths at 543 (g-QDs) and at 627 nm (r-QDs). The second comprises gold nanoparticles acting as a quencher. In the absence of the target, g-QD-N1 and r-QD-N2 are stable due to the fluorescence stability. With the addition of microRNA-122 and microRNA-199a/b-3p, g-QD-N1 and r-QD-N2 are conjugated to the surface of AuNP-S1/S2 through base complementary pairing. As a result, fluorescence resonance energy transfer (FRET) occurs, resulting in a decrease at 550 nm and 635 nm respectively, which can realize the simultaneous detection of two different microRNAs. Detection is achieved within 50 min. The detection limits (3σ/k) are 0.2 nM for microRNA-122 and 0.5 nM for microRNA-199a/b-3p. The clinical applicability of the assay was demonstrated by detecting microRNAs in human serum and different cell lysates. [ABSTRACT FROM AUTHOR]
- Subjects :
- *QUANTUM dots
*FLUORESCENCE resonance energy transfer
*BASE pairs
Subjects
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 187
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 141879796
- Full Text :
- https://doi.org/10.1007/s00604-020-4117-y