A loop‐mediated isothermal amplification assay for the detection of Dahlia mosaic caulimovirus in Dahlia (Dahlia variabilis).

Dahlia mosaic virus (DMV), an aphid‐transmitted caulimovirus in the family Caulimoviridae, is an important pathogen of dahlia (Dahlia variabilis). Development of rapid, and sensitive diagnostic tools is essential in surveillance and management of DMV. A loop‐mediated isothermal amplification (LAMP) assay was developed for DMV detection. The LAMP assay targets the reverse transcriptase region of the DMV genome and is optimal at a temperature of 60°C and run time of 60 min, and amplification was detected through fluorescence detection and by agarose gel electrophoresis. The assay detected DMV in DNA concentration as low as 10−3 ng. To the best of our knowledge, this is the first report of a LAMP assay for the detection of DMV, a plant DNA virus. This assay will be useful in rapid and sensitive detection of DMV and in reducing the virus incidence through production of virus‐free planting material. [ABSTRACT FROM AUTHOR]