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OffScan: a universal and fast CRISPR off-target sites detection tool.
- Source :
-
BMC Genomics . 3/5/2020 Supplement 1, Vol. 21, p1-6. 6p. 1 Color Photograph, 2 Charts, 2 Graphs. - Publication Year :
- 2020
-
Abstract
- Background: The Type II clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) is a powerful genome editing technology, which is more and more popular in gene function analysis. In CRISPR/Cas, RNA guides Cas nuclease to the target site to perform DNA modification. Results: The performance of CRISPR/Cas depends on well-designed single guide RNA (sgRNA). However, the off-target effect of sgRNA leads to undesired mutations in genome and limits the use of CRISPR/Cas. Here, we present OffScan, a universal and fast CRISPR off-target detection tool. Conclusions: OffScan is not limited by the number of mismatches and allows custom protospacer-adjacent motif (PAM), which is the target site by Cas protein. Besides, OffScan adopts the FM-index, which efficiently improves query speed and reduce memory consumption. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GENOME editing
*DNA
*RNA
Subjects
Details
- Language :
- English
- ISSN :
- 14712164
- Volume :
- 21
- Database :
- Academic Search Index
- Journal :
- BMC Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 142083687
- Full Text :
- https://doi.org/10.1186/s12864-019-6241-9