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Ampelopsin Induces DR5-Mediated Apoptotic Cell Death in EBV-Infected Cells through the p38 Pathway.
- Source :
-
Nutrition & Cancer . 2020, Vol. 72 Issue 3, p489-494. 6p. 3 Charts, 1 Graph. - Publication Year :
- 2020
-
Abstract
- Ampelopsin (AMP) is a well-known flavonoid that exerts a number of biological and pharmacological effects including anticancer effects against several cancer cell lines. In this study, we investigated the anticancer activity of AMP against Epstein-Barr virus (EBV)-positive cells and its mechanism of action. Our results showed that AMP dose-dependently inhibited cell viability and induced apoptotic cell death in EBV-positive cells without cytotoxicity in EBV-negative cells. In particular, AMP induced caspase-8 dependent apoptosis via upregulation of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and death receptor (DR5). Knockdown of DR5 by RNA interference blocked AMP-induced apoptosis. Furthermore, AMP dose-dependently activated p38 mitogen-activated protein kinases (MAPKs) in EBV-positive cells. Additionally, SB203580 (a p38-MAPK inhibitor) effectively inhibited apoptotic cell death. These results demonstrate that treatment with AMP induces the apoptosis of EBV-positive cells through upregulation of TRAIL/DR5 and activation of p38 signaling. Therefore, these results provide experimental information for developing AMP as a new therapeutic drug against EBV-positive cancer. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01635581
- Volume :
- 72
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Nutrition & Cancer
- Publication Type :
- Academic Journal
- Accession number :
- 142124105
- Full Text :
- https://doi.org/10.1080/01635581.2019.1639778