Glycodelin is internalized by peripheral monocytes.

• Glycodelin - with or without the carbohydrate structure – was preferentially bound mainly to peripheral monocytes. • More monocytes were labelled with deglycosylated glycodelin compared to native glycodelin or recombinant glycodelin. • Glycodelin was internalized and entered the nucleus of the peripheral monocytes. Glycodelin is produced by the endometrial cells during the luteal phase and first trimester of pregnancy and plays a role in the regulation of the endometrial immunology. However, the molecular connection between glycodelin and the maternal immune system is not clear. To better understand the possible physiological interaction between the endometrium and the maternal immune system, we investigated (1) whether glycodelin binds to mainly peripheral monocytes, and in case (2) whether the binding to the membrane only depends on the protein backbone or a carbohydrate structure is needed, and in case (3) whether glycodelin is internalized after binding to the membrane. We demonstrated that glycodelin - with or without the carbohydrate structure – was preferentially bound and internalized to peripheral monocytes. Surprisingly, we found signals in the nucleus of the monocytes indicating a potential regulating effect of glycodelin may be exerted through the nucleus. However, further studies should be performed to confirm this finding. [ABSTRACT FROM AUTHOR]