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Facile preparation of FITC-modified silicon nanodots for ratiometric pH sensing and imaging.
- Source :
-
Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy . Jun2020, Vol. 234, pN.PAG-N.PAG. 1p. - Publication Year :
- 2020
-
Abstract
- A ratiometric fluorescent pH sensor was facilely constructed by covalent modification of amino-terminated silicon nanodots (SiND) with pH-sensitive fluorescein isothiocyanate (FITC). After optimization, the SiND-FITC(40:1) material with a SiND:FITC initial mass ratio of 40:1 was selected for the sensing of hydrogen ions. It was observed that the material inherits the unique features of SiND and FITC, and there is significant improvement of SiND acid−base stability, which is a favorable factor in terms of providing fluorescence reference signal. The SiND-FITC(40:1) material displays not only high pH sensitivity, but also good stability and anti-interference ability, and the response process is highly reversible. Deploying the SiND-FITC(40:1) material, we have made available a simple, sensitive, and precise approach for pH sensing. In aqueous solutions, the I 517 /I 466 fluorescence intensity ratio of SiND-FITC(40:1) increases linearly in the pH range of 5.40–7.76. This dual emission nanosensor was successfully applied for pH sensing and cellular fluorescence imaging. Unlabelled Image • Water-soluble silicon nanodots could be synthesized in large-scale. • Facile covalent modification of the silicon nanodots with FITC was achieved. • An efficacious method for ratiometric pH sensing and imaging was proposed. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13861425
- Volume :
- 234
- Database :
- Academic Search Index
- Journal :
- Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- 142636485
- Full Text :
- https://doi.org/10.1016/j.saa.2020.118276