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Electrolyte and transporter responses to angiotensin II induced hypertension in female and male rats and mice.
- Source :
-
Acta Physiologica . May2020, Vol. 229 Issue 1, p1-12. 12p. 5 Graphs. - Publication Year :
- 2020
-
Abstract
- Aim: Sexual dimorphisms are evident along the nephron: Females (F) exhibit higher ratios of renal distal to proximal Na+ transporters' abundance, greater lithium clearance (CLi) more rapid natriuresis in response to saline infusion and lower plasma [K+] vs. males (M). During angiotensin II infusion hypertension (AngII‐HTN) M exhibit distal Na+ transporter activation, lower proximal and medullary loop transporters, blunted natriuresis in response to saline load, and reduced plasma [K+]. This study aimed to determine whether responses of F to AngII‐HTN mimicked those in M or were impacted by sexual dimorphisms evident at baseline. Methods: Sprague Dawley rats and C57BL/6 mice were AngII infused via osmotic minipumps 2 and 3 weeks, respectively, and assessed by metabolic cage collections, tail‐cuff sphygmomanometer, semi‐quantitative immunoblotting of kidney and patch‐clamp electrophysiology. Results: In F rats, AngII‐infusion increased BP to 190 mm Hg, increased phosphorylation of cortical NKCC2, NCC and cleavage of ENaC two to threefold, increased ENaC channel activity threefold and aldosterone 10‐fold. K+ excretion increased and plasma [K+] decreased. Evidence of natriuresis in F included increased urine Na+ excretion and CLi, and decreased medullary NHE3, NKCC2 and Na,K‐ATPase abundance. In C57BL/6 mice, AngII‐HTN increased abundance of distal Na+ transporters, suppressed proximal‐medullary transporters and reduced plasma [K+] in both F and M. Conclusion: Despite baseline sexual dimorphisms, AngII‐HTN provokes similar increases in BP, aldosterone, distal transporters, ENaC channel activation and K+ loss accompanied by similar suppression of proximal and loop Na+ transporters, natriuresis and diuresis in females and males. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANGIOTENSIN II
*SPRAGUE Dawley rats
*SEXUAL dimorphism
*MICE
*EXCRETION
Subjects
Details
- Language :
- English
- ISSN :
- 17481708
- Volume :
- 229
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Acta Physiologica
- Publication Type :
- Academic Journal
- Accession number :
- 142704842
- Full Text :
- https://doi.org/10.1111/apha.13448