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MicroRNA-1271-5p inhibits cell proliferation and enhances radiosensitivity by targeting CDK1 in hepatocellular carcinoma.
- Source :
-
Journal of Biochemistry . May2020, Vol. 167 Issue 5, p513-524. 12p. - Publication Year :
- 2020
-
Abstract
- This study aims to determine whether miR-1271-5p inhibits cell proliferation and enhances the radiosensitivity by targeting cyclin-dependent kinase 1 (CDK1) in hepatocellular carcinoma (HCC). Its expression levels in the HCC cell lines were significantly lower than those in normal human liver cell line. Bioinformatics analysis indicated CDK1 was a potential target of miR-1271-5p. Dual-Luciferase Reporter Assay confirmed that CDK1 is a direct target gene of miR-1271-5p. With overexpression of miR-1271-5p in SMMC-7721 and HuH-7 cells, cell proliferation was decreased, radiosensitivity was enhanced, cell cycle distribution was altered and the growth of transplanted tumours in nude mice was significantly reduced. miR-1271-5p overexpression enhanced radiosensitivity, which could be reduced by CDK1 overexpression. Overall, our findings suggested that miR-1271-5p inhibits cell proliferation and enhances the radiosensitivity of HCC cell lines by targeting CDK1. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CELL proliferation
*HEPATOCELLULAR carcinoma
*CELL cycle
*LIVER cells
*CELL lines
Subjects
Details
- Language :
- English
- ISSN :
- 0021924X
- Volume :
- 167
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 142976156
- Full Text :
- https://doi.org/10.1093/jb/mvz114