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Target-fueled catalytic hairpin assembly for sensitive and multiplex microRNA detection.
- Source :
-
Analytical & Bioanalytical Chemistry . May2020, Vol. 412 Issue 13, p3019-3027. 9p. - Publication Year :
- 2020
-
Abstract
- As a typical strand displacement–based DNA circuit, the catalytic hairpin assembly (CHA) has the potential to transduce and amplify signals for analytical applications, but little practice has been fulfilled in Luminex-based multiple microRNAs (miRNAs) detection. Here, we proposed a target-fueled CHA-based platform for sensitive and multiple miRNAs detection, by virtue of the multiplex characteristic of the Luminex xMAP platform. The cyclic use of target miRNA, which forms a substantial amount of H1-H2 duplexes, has amplified the fluorescent response to achieve sensitive sensing. Key experimental conditions including hairpin probe concentrations, reaction temperature, and concentration of SA-PE were optimized. Liver tumor–related miRNA-21, miRNA-122, and miRNA-222 could be simultaneously detected with LOD of 2 pM. Overall, the proposed method first combined CHA with the Luminex xMAP system to construct a sensitive sensing platform suitable for multiple miRNAs detection in real sample analysis, which could potentially be applied in biomedical research and clinical diagnosis. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HAIRPIN (Genetics)
*MICRORNA
*DNA
*MEDICAL research
Subjects
Details
- Language :
- English
- ISSN :
- 16182642
- Volume :
- 412
- Issue :
- 13
- Database :
- Academic Search Index
- Journal :
- Analytical & Bioanalytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 143057041
- Full Text :
- https://doi.org/10.1007/s00216-020-02531-w