长链非编码RNA LINC01296对胃癌细胞增殖的影响及其机制.

Objective To observe the effect and mechanism of long non-coding RNA ( LINC01296) on the proliferation of gastric cancer cells GESl. Methods We collected the human gastric cancer cell lines SGC7901, BGC823, and normal gastric epithelial cells GES1. The expression of LINC01296 in the above cells was detected by real-time quantitative PCR. SGC-7901 and BGC-823 cells were divided into two parts, which were transfected with small interfering RNA (siRNA) and control siRNA (siN C), respectively, after 24 hours, the cells were collected. CCK-8 assay was used to detect the OD value at a wavelength of 450 nm ( OD value). The clone formation assay was used to detect the number of clone formation. The subcellular fractionation assay was used to observe the distribution of LINC01296 in gastric cancer cells. RNA immunoprecipitation (RIP) experiment was used to observe the interaction of LINC01296 with EZH2 and SUZ12. Results The expression levels of LINC01296 in SGC7901, BGC823 and GES1 cells was 4. 039 ± 1. 64, 5. 33 ± 1. 32, and 1. 41 ± 0. 01, respectively, which indicated that the expression level of LINC01296 in SGC7901 and BGC823 cells was higher than that of GES1 cells. The OD value of SGC7901 cells transfected with siRNA and siNC were 1. 20 ± 0. 03 and 1. 38 ± 0. 02, respectively, and the number of clone formations were 112. 56 ± 5. 22 and 256. 14 ± 6. 42, respectively( both P < 0. 05) ; The OD values of BGC823 cells transfected with siRNA and siNC were 1. 50 ± 0. 04 and 1. 73 ± 0. 22, respectively. The number of clones formations was 98. 21 ± 5. 56 and 203. 76 ± 5. 31 ( both P < 0. 05) . The relative expression of LINC01296 in the nuclei of SGC7901 and BGC823 cells was higher than that of cytoplasm (both P < 0. 05). RIP experiments showed that LINC01296 can be combined with EZH2 and SUZ12. Conclusion LINC01296 is highly expressed in the gastric cancer and could promote the proliferation of gastric cancer cells through combining with EZH2 and SUZ12. [ABSTRACT FROM AUTHOR]