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Group O plasma as a media supplement for CAR-T cells and other adoptive T-cell therapies.
- Source :
-
Transfusion . May2020, Vol. 60 Issue 5, p1004-1014. 11p. 1 Diagram, 4 Charts, 3 Graphs. - Publication Year :
- 2020
-
Abstract
- <bold>Background: </bold>Most chimeric antigen receptor T (CAR-T) cells and other adoptive T-cell therapies (ACTs) are currently manufactured by ex vivo expansion of patient lymphocytes in culture media supplemented with human plasma from group AB donors. As lymphocytes do not express A or B antigens, the isoagglutinins of non-AB plasmas are unlikely to cause deleterious effects on lymphocytes in culture.<bold>Study Design and Methods: </bold>Seeding cultures with peripheral blood mononuclear cell (PBMNC) concentrates from group A1 donors and using a CAR-T culture protocol, parallel cultures were performed, each with unique donor plasmas as media supplements (including group O plasmas with high-titer anti-A and group AB plasmas as control). An additional variable, a 3% group A1 red blood cell (RBC) spike, was added to simulate a RBC-contaminated PBMNC collection. Cultures were monitored by cell count, viability, flow cytometric phenotype, gene expression analysis, and supernatant chemokine analysis.<bold>Results: </bold>There was no difference in lymphocyte expansion or phenotype when cultured with AB plasma or O plasma with high-titer anti-A. Compared to controls, the presence of contaminating RBCs in lymphocyte culture led to poor lymphocyte expansion and a less desirable phenotype-irrespective of the isoagglutinin titer of the plasma supplement used.<bold>Conclusions: </bold>This study suggests that ABO incompatible plasma may be used as a media supplement when culturing cell types that do not express ABO antigens-such as lymphocytes for CAR-T or other ACT. The presence of contaminating RBCs in culture was disadvantageous independent of isoagglutinin titer. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ERYTHROCYTES
*MASS media
*CHIMERIC antigen receptors
*BLOOD cells
*PLASMA confinement
*ABO blood group system
*FLOW cytometry
*CELL culture
*IMMUNIZATION
*MONONUCLEAR leukocytes
*HLA-B27 antigen
*CULTURE media (Biology)
*BLOOD plasma
*CELL receptors
*IMMUNOLOGY technique
*RESEARCH funding
*T cells
*BLOOD
Subjects
Details
- Language :
- English
- ISSN :
- 00411132
- Volume :
- 60
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Transfusion
- Publication Type :
- Academic Journal
- Accession number :
- 143302697
- Full Text :
- https://doi.org/10.1111/trf.15745