Alterations in peripheral blood B cells in systemic lupus erythematosus patients with renal insufficiency.

• CD38+ B cells are predominant among SLE circulating B cells. • Alterations of MBD2, DNMT1 and APRIL gene expression in B cells are associated with SLE severity. • SLE patients with RI display significant changes in circulating B cell population. Systemic lupus erythematosus (SLE) is one of the autoimmune diseases, believed to be closely related to hyperactivity of B cells, overproduction of autoantibodies and immune complex formation and deposition in affected tissue. The autoreactive inflammation leads to multiorgan damage with kidney dysfunction in the forefront. Studies on lupus nephritis (LN), affecting the majority of SLE patients, are mainly focused on cells causing local inflammation. The aim of our work was to detect alterations in more accessible peripheral blood B cells in the course of SLE focusing on the influence of renal insufficiency (RI) on those parameters. We performed a comprehensive flow cytometry analysis of B cell subpopulations, analyzed gene expression patterns with qPCR, and examined serum cytokine levels with multiplex cytokine/chemokine assay. We discovered distribution of specific B cell subsets, especially CD38+ cells, plasmablasts, associated with the presence and severity of the disease. Changes in expression of MBD2 , DNMT1 and APRIL genes were not only associated with activity of SLE but also were significantly changed in patients with RI. All these results shed new light on the role of circulating B cells, their subpopulations, function, and activity in the SLE with kidney manifestation. [ABSTRACT FROM AUTHOR]