甲基丙烯酸钠修饰光交联海藻酸盐水凝胶支架的制备和表征.

BACKGROUND: Photocross linked alginate hydrogel has been a popular bone tissue engineering material because of its excellent biocompatibility and minimally invasive injection, but there are still problems such as insufficient strength and poor cell adhesion. OBJECTIVE: To construct the negatively charged hydrogels by introducing sodium methacrylate into photocrosslinked alginate hydrogels, and to explore the changes in its physical performance and cell adhesion. METHODS: After preparation of methacrylated alginate by reacting sodium alginate with 2-aminoethyl melhacrylate, methacrylated alginate, photoinitiator and sodium methacrylate (0, 20, 40, 60 mmol/L) were homogeneously mixed. The negatively charged photocrosslinked alginate hydrogels were prepared under ultraviolet light. The functional groups of the hydrogels were analyzed by fourier transform infrared spectroscopy. The surface morphology of the hydrogels was observed by scanning electron microscopy and the swelling ratio was measured. MC3T3-E1 cells were cultured with each group of hydrogels for 48 hours, and the cytotoxicity of the hydrogels was investigated by cell counting kit-8assay. MC3T3-E1 cells were seeded on the surface of each group of hydrogels. The early adhesion of the cells was observed by live/dead staining at the 4th hour, and cell spreading was observed on the 3thday. RESULTS AND CONCLUSION: (1) Fourier transfom infrared spectroscopy demonstrated that the introduction of sodium methacrylate could lead to a new peak at wavenumber of about 1600 cm-1 in the hydrogel infrared wave, which was from the sodium methacrylate. (2) Scanning electron microscope observed that the density of the negatively charged photocrosslinked alginate hydrogels increased and the pore size of the gels decreased with augment of concentrations of sodium methacrylate. (3) The swelling ratio of the hydrogel decreased with the increase of the concentration of sodium methacrylate. (4) The live/dead staining revealed that the cells grew well on the surface of each hydrogel, and the cell viability reached above 95%. The cell counting kit-8 assay results showed that the negatively charged photocrosslinked alginate hydrogels had no cytotoxicity. (5) The early cell adhesion rate increased gradually and the cell extension became better with the increase of concentration of sodium methacrylate. (6) In summary, the introduction of sodium methacryl into pholocrosslinked alginate hydrogels can adjust its physical properties and significantly improve its cell adhesion. [ABSTRACT FROM AUTHOR]