Modeling Kinetics of the Water Extraction of Protein from Caragana korshinskii Kom.

A two-step extraction method was used to extract proteins from Caragana korshinskii Kom., including a NaOH solution extraction followed by a water extraction. A power-law model, three-site kinetic extraction model, and second-order model were utilized to investigate the mechanism of the water extraction process and the key factors affecting the protein yield. The experimental data fitted well with the three-site kinetic model, indicating that the water extraction process included washing and faster and slower stages. In addition, the slower stage was the rate-limiting step. For the water extraction process, the protein yield was increased by decreasing the particle size, increasing the NaOH concentration, or raising the extraction temperature, among which the extraction temperature was the critical factor for controlling the protein yield. [ABSTRACT FROM AUTHOR]