Effects of cryopreservation on antiviral responses of primary airway epithelial cells.

Keywords: airway epithelium; cryopreservation; human rhinovirus EN airway epithelium cryopreservation human rhinovirus 1486 1489 4 06/01/20 20200601 NES 200601 To the Editor, Human epithelial cell lines (eg, A549 and BEAS-2B cells), as well as primary nasal and bronchial epithelial cells, have been used extensively to explore the role of virus-host interactions in respiratory allergic diseases.[1] In vitro cell cultures of primary airway epithelial cells from patients are a key model for these studies. In RV-infected cells, 45 genes were differentially expressed (36 higher and 9 lower in fresh compared to cryopreserved cells) (Figure C, Table S2B). In the last decades, epithelial infection models have evolved from using cell lines[5] to submerged primary cells[6] to air-liquid interphase differentiated cells.[7] At the same time, utilization of cryopreserved cells or tissues has increased considerably. [Extracted from the article]