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Ginkgolide B inhibits hydrogen peroxide-induced apoptosis and attenuates cytotoxicity via activating the PI3K/Akt/mTOR signaling pathway in H9c2 cells.
- Source :
-
Molecular Medicine Reports . Jul2020, Vol. 22 Issue 1, p310-316. 7p. - Publication Year :
- 2020
-
Abstract
- Ginkgolide B (GB) is a diterpene lactone found in the leaves of the traditional Chinese medicinal plant Ginkgo that has been shown to have various pharmacological effects. However, the anti-apoptotic properties of GB in cardiovascular disease remain poorly understood. The present study aimed to investigate the effect of GB on hydrogen peroxide-induced cell injury in cardiac H9c2 cells, and to further clarify its protective mechanism of action. An in vitro hydrogen peroxide-treated H9c2 cell model was used in order to mimic myocardial ischemia-reperfusion (I/R) injury. Cell viability was assessed by the Cell Counting Kit-8 assay. The induction of apoptosis was determined by flow cytometry and staining was performed using Hoechst 33342. In addition, the effect of GB on the expression levels of apoptosis-associated proteins was evaluated by western blot analysis. The present study demonstrated that GB protected against hydrogen peroxide-induced cytotoxicity and cell apoptosis in H9c2 cardiac cells. GB upregulated the expression level of the anti-apoptotic protein Bcl-2 and downregulated the expression levels of the pro-apoptotic proteins cleaved caspase-3 and Bax in hydrogen peroxide-treated H9c2 cells. The molecular mechanism underlying the anti-apoptotic effects of GB was subsequently detected. GB pretreatment activated the PI3K/Akt/mTOR signaling pathway and caused an increase in the phosphorylation levels of Akt and mTOR in hydrogen peroxide-treated H9c2 cells. These results revealed that GB inhibited hydrogen peroxide-induced apoptosis in H9c2 cells via activation of the PI3K/Akt/mTOR signaling pathway. These findings indicate the potential therapeutic benefits of GB in the treatment of myocardial I/R injury. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17912997
- Volume :
- 22
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Molecular Medicine Reports
- Publication Type :
- Academic Journal
- Accession number :
- 143609699
- Full Text :
- https://doi.org/10.3892/mmr.2020.11099