幽门螺杆菌感染性胃癌组织中cyclinD1、MMP-9 的表达及其临床意义.

Objective: To investigate the expression of cyclinD1 and matrix metalloproteinase-9 (MMP-9) in helicobacter pylori (HP) infected gastric carcinoma and its clinical significance. Methods: 80 patients with gastric cancer who were treated in Lanzhou University First Hospital from December 2016 to June 2018 were selected and their pathological tissues were collected. C-14 breath test and modified Giemsa staining were used to detect the status of HP infection. Immunohistochemistry was used to detect the expression of cyclinD1 and MMP-9 in gastric cancer tissues. The relationship between HP infection and cyclinD1, MMP-9 expression and clinicopathological features of patients with gastric cancer was analyzed. The correlation between HP infection and cyclinD1, MMP-9 expression in patients with gastric cancer was analyzed. Results: In 80 patients with gastric cancer, 56 cases were positive for HP infection (70.00%) and 24 cases were negative (30.00%). The positive rate of HP infection in gastric cancer patients with lymph node metastasis and depth of invasion with T3+T4 was higher than that in gastric cancer patients without lymph node metastasis and depth of invasion with T1+T2 (P<0.05). In 80 patients with gastric cancer, 45 cases were positive for cyclinD1 expression (56.25%), 35 cases were negative expression (43.75%), 65 cases were positive for MMP-9 expression (81.25%), 15 cases were negative expression (18.75%). The positive expression rates of cyclinD1 and MMP-9 in gastric cance patients with TNM stage for III+IV stage, low differentiation, lymph node metastasis and depth of invasion with T3+T4 were significantly higher than that of TNMstage for I+II stage, moderately high differentiation, no lymph node metastasis and depth of invasion with T1+T2 (P<0.05). The positive rate of cyclinD1 and MMP-9 in HP positive group was significantly higher than that in HP negative group (P<0.05). Pearson correlation analysis showed that HP infection was positively correlated with cyclinD1 and MMP-9 expression in patients with gastric cancer (P<0.05). Conclusion: HP infection in patients with gastric cancer is related to lymph node metastasis and depth of invasion. The expression of cyclinD1 and MMP-9 is related to TNMclinical stage, differentiation, lymph node metastasis and depth of invasion.Moreover, HP infection is positively correlated with cyclinD1 and MMP-9 expression in gastric cancer patients. [ABSTRACT FROM AUTHOR]