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Osa-miR162a fine-tunes rice resistance to Magnaporthe oryzae and Yield.
- Source :
-
Rice (19398425) . 6/10/2020, Vol. 13 Issue 1, p1-13. 13p. - Publication Year :
- 2020
-
Abstract
- MicroRNAs (miRNAs) play essential roles in rice immunity against Magnaporthe oryzae, the causative agent of rice blast disease. Here we demonstrate that Osa-miR162a fine-tunes rice immunity against M. oryzae and yield traits. Overexpression of Osa-miR162a enhances rice resistance to M. oryzae accompanying enhanced induction of defense-related genes and accumulation of hydrogen peroxide (H2O2). In contrast, blocking Osa-miR162 by overexpressing a target mimic of Osa-miR162a enhances susceptibility to blast fungus associating with compromised induction of defense-related gene expression and H2O2 accumulation. Moreover, the transgenic lines overexpressing Osa-miR162a display decreased seed setting rate resulting in slight reduced yield per plant, whereas the transgenic lines blocking Osa-miR162 show an increased number of grains per panicle, resulting in increased yield per plant. Altered accumulation of Osa-miR162 had a limited impact on the expression of rice Dicer-like 1 (OsDCL1) in these transgenic lines showing normal gross morphology, and silencing of OsDCL1 led to enhanced resistance to blast fungus similar to that caused by overexpression of Osa-miR162a, suggesting the involvement of OsDCL1 in Osa-miR162a-regulated resistance. Together, our results indicate that Osa-miR162a is involved in rice immunity against M. oryzae and fine-tunes resistance and yield. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HYDROGEN peroxide
*RICE blast disease
*MICRORNA
Subjects
Details
- Language :
- English
- ISSN :
- 19398425
- Volume :
- 13
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Rice (19398425)
- Publication Type :
- Academic Journal
- Accession number :
- 143699564
- Full Text :
- https://doi.org/10.1186/s12284-020-00396-2