Leucocyte‐ and platelet‐rich fibrin regulates expression of genes related to early wound healing in human gingival fibroblasts.

Background: Leucocyte‐ and platelet‐rich fibrin (L‐PRF) is a blood‐derived biomaterial rich in leucocytes and platelets embedded in a high‐density fibrin network that can be compressed into a membrane and used in surgical applications to stimulate tissue regeneration and wound healing, especially in oral cavity. This study aimed to determine the combined effects of the growth factors and cells present in L‐PRF on fibroblasts that directly face the L‐PRF membranes placed during surgical procedures. Methods: The effect of L‐PRF from six donors on the expression of 84 key wound healing genes in normal human gingival fibroblasts was tested by RT‐qPCR. Results: L‐PRF significantly regulated the expression of 33 fibroblast genes (39%), including interleukins, myofibroblast‐, extracellular matrix‐ and angiogenesis‐associated genes, and matrix metalloproteinase‐1 and −3. L‐PRF regulated fibroblast gene expression both time‐ and dose‐dependently, and the effects were mediated by mitogen‐activated protein kinases ERK1/2, JNK and p38. L‐PRF also stimulated fibroblast wound closure and promoted the ability of fibroblasts to induce endothelial tube formation. L‐PRF‐induced gene expression changes in fibroblast were similar to those observed in early human and pig wounds. Conclusions: This study provides new insights into the biological mechanism by which L‐PRF regulates key gingival fibroblast functions important in wound healing. [ABSTRACT FROM AUTHOR]