Back to Search
Start Over
Perfusion-based co-culture model system for bone tissue engineering.
- Source :
-
AIMS Bioengineering . 2020, Vol. 7 Issue 2, p91-105. 15p. - Publication Year :
- 2020
-
Abstract
- In this work, we report on a perfusion-based co-culture system that could be used for bone tissue engineering applications. The model system is created using a combination of Primary Human Umbilical Vein Endothelial Cells (HUVECs) and osteoblast-like Saos-2 cells encapsulated within a Gelatin Methacrylate (GelMA)-collagen hydrogel blend contained within 3D printed, perfusable constructs. The constructs contain dual channels, within a custom-built bioreactor, that were perfused with osteogenic media for up to two weeks in order to induce mineral deposition. Mineral deposition in constructs containing only HUVECs, only Saos-2 cells, or a combination thereof was quantified by microCT to determine if the combination of endothelial cells and bone-like cells increased mineral deposition. Histological and fluorescent staining was used to verify mineral deposition and cellular function both along and between the perfused channels. While there was not a quantifiable difference in the amount of mineral deposited in Saos-2 only versus Saos-2 plus HUVEC samples, the location of the deposited mineral differed dramatically between the groups and indicated that the addition of HUVECs within the GelMA matrix allowed Saos-2 cells, in diffusion limited regions of the construct, to deposit bone mineral. This work serves as a model on how to create perfusable bone tissue engineering constructs using a combination of 3D printing and cellular co-cultures. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 23751495
- Volume :
- 7
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- AIMS Bioengineering
- Publication Type :
- Academic Journal
- Accession number :
- 144298734
- Full Text :
- https://doi.org/10.3934/bioeng.2020009