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MiR-345-5p inhibits tumorigenesis of papillary thyroid carcinoma by targeting SETD7.
- Source :
-
Archives of Medical Science . 2020, Vol. 16 Issue 4, p888-897. 10p. - Publication Year :
- 2020
-
Abstract
- <bold>Introduction: </bold>This study aimed to explore the effects of miR-345-5p on papillary thyroid carcinoma (PTC) and uncover the possible mechanisms.<bold>Material and Methods: </bold>MiR-345-5p and SETD7 mRNA levels were analyzed by quantitative real-time PCR and SETD7 protein level was measured by Western blot. The viability, colony formation ability and apoptosis of PTC cells were measured with CCK-8, soft agar colony formation and flow cytometry assay, respectively. Luciferase reporter assay was used to identify miR-345-5p's target.<bold>Results: </bold>Compared to neighboring normal tissues, there was lower miR-345-5p expression and higher SETD7 expression in PTC tissues. Moreover, Spearman's correlation analysis indicated that there was a negative correlation between miR-345-5p and SETD7 expression in PTC tissues. MiR-345-5p mimics inhibited the viability and colony formation of TPC1 and B-CPAP cells and promoted apoptosis, whereas anti-miR-345-5p promoted PTC cell proliferation and inhibited apoptosis. SETD7 was confirmed to be a direct target of miR-345-5p through target scan analysis and luciferase reporter assay. Additionally, overexpression of SETD7 promoted the viability and colony formation of TPC1 and B-CPAP cells and inhibited apoptosis, whereas downregulation of SETD7 by shRNAs had opposite effects on PTC cells. Furthermore, overexpression of SETD7 attenuated the miR-345-5p induced anti-tumor effects on PTC cells.<bold>Conclusions: </bold>MiR-345-5p exhibited suppressive effects on PTC via targeting SETD7. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17341922
- Volume :
- 16
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Archives of Medical Science
- Publication Type :
- Academic Journal
- Accession number :
- 144308381
- Full Text :
- https://doi.org/10.5114/aoms.2019.83823