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Design of a two-photon fluorescent probe for ratiometric imaging of endogenous hypochlorite in mitochondria.
- Source :
-
Dyes & Pigments . Oct2020, Vol. 181, pN.PAG-N.PAG. 1p. - Publication Year :
- 2020
-
Abstract
- Hypochlorite (ClO−) is associated with complex physiological activities in the living system. Therefore, it is necessary to develop a tool for visualizing ClO− at the cellular and subcellular levels. We herein presented a two-photon fluorescent probe MCL with dual response sites and ratiometric response to ClO−. The response sites of two oxathiolane rings endowed probe MCL with superior selectivity for ClO− over other analytes. The fast response and high sensitivity of MCL to ClO− were also well demonstrated. In addition, probe MCL was confirmed to possess accurate mitochondrial targeting ability through a co-localization study. The good two-photon sensing performance of MCL for exogenous and LPS-induced endogenous ClO− in living cells was validated by dual-color imaging. Moreover, we employed the probe to successfully monitor endogenous ClO− in H 2 S-stimulated cells by dual-channel mode for the first time. A two-photon fluorescent probe with dual response sites for ratiometric imaging of mitochondrial hypochlorite in living cells has been developed. Image 1 • A ratiometric two-photon fluorescent probe was designed for monitoring ClO−. • The probe showed ultrafast response and high selectivity for ClO− detection. • Dual-channel imaging of exogenous and endogenous ClO− in mitochondria was obtained. • The probe was used for dual-color imaging of H 2 S-induced ClO− in cells for the first time. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FLUORESCENT probes
*MITOCHONDRIA
*IMAGE
Subjects
Details
- Language :
- English
- ISSN :
- 01437208
- Volume :
- 181
- Database :
- Academic Search Index
- Journal :
- Dyes & Pigments
- Publication Type :
- Academic Journal
- Accession number :
- 144460353
- Full Text :
- https://doi.org/10.1016/j.dyepig.2020.108548