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High expression of aldehyde dehydrogenase 1 and tissue necrosis factor alpha may relate to chronic infection of buffalo mammary gland.
- Source :
-
Animal Biotechnology . Jun2020, Vol. 31 Issue 3, p276-281. 6p. 2 Color Photographs, 1 Chart, 2 Graphs. - Publication Year :
- 2020
-
Abstract
- Aldehyde dehydrogenase 1 (ALDH1) and hepatocyte nuclear factor 4A (HNF4A) are the putative mammary stem cell markers. Tissue necrosis factor alpha (TNFA) is involved in inflammation-associated carcinogenesis and cell proliferation. In this study, the gene expression profile of ALDH1, HNF4A and TNFA of buffalo mammary tissue using real-time quantitative PCR (RT-qPCR). Analysis of RT-qPCR data revealed that the relative expression (log2 fold change) of ALDH1 and TNFA during mastitis (vs. lactation) was increased (P <.05) by 2.98 and 4.71, respectively. The relative expression (log2 fold change; −7.39) of stem cell marker, HNF4A was decreased (P <.05) during mastitis. Histological analysis of mammary tissue during mastitis showed thickening of stroma and occasionally hyperplasia, predominantly in prepubertal and non-lactating animals. Although, the level of expression of these genes may vary, depending upon the physiological stage of the animals, however expression of ALDH1 and TNFA was high during mastitis. A systematic study on large samples of buffalo mammary tissue with appropriate comparisons needs to be evaluated with these markers for prognosis of buffalo mammary health. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10495398
- Volume :
- 31
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Animal Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 144637378
- Full Text :
- https://doi.org/10.1080/10495398.2019.1579099