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CrAssphage as a Novel Tool to Detect Human Fecal Contamination on Environmental Surfaces and Hands.
- Source :
-
Emerging Infectious Diseases . Aug2020, Vol. 26 Issue 8, p1731-1739. 9p. 2 Diagrams, 4 Charts. - Publication Year :
- 2020
-
Abstract
- CrAssphage is a recently discovered human gut-associated bacteriophage. To validate the potential use of crAssphage for detecting human fecal contamination on environmental surfaces and hands, we tested stool samples (n = 60), hand samples (n = 30), and environmental swab samples (n = 201) from 17 norovirus outbreaks for crAssphage by real-time PCR. In addition, we tested stool samples from healthy persons (n = 173), respiratory samples (n = 113), and animal fecal specimens (n = 68) and further sequenced positive samples. Overall, we detected crAssphage in 71.4% of outbreak stool samples, 48%-68.5% of stool samples from healthy persons, 56.2% of environmental swabs, and 60% of hand rinse samples, but not in human respiratory samples or animal fecal samples. CrAssphage sequences could be grouped into 2 major genetic clusters. Our data suggest that crAssphage could be used to detect human fecal contamination on environmental surfaces and hands. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10806040
- Volume :
- 26
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Emerging Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 144704901
- Full Text :
- https://doi.org/10.3201/eid2608.200346