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A four-microorganism three-step fermentation process for producing medium-chain-length polyhydroxyalkanoate from starch.
- Source :
-
3 Biotech . 7/23/2020, Vol. 10 Issue 8, p1-9. 9p. - Publication Year :
- 2020
-
Abstract
- In this study, a four-microorganism three-step fermentation process was established for producing medium-chain-length polyhydroxyalkanoate (mcl-PHA) from starch, which was used as the sole carbon source. The four microorganisms used for this process were Aspergillus niger, Saccharomyces cerevisiae L2612, Acetobacter orientalis, and Pseudomonas putida KT2440-acs. The initial carbon source starch concentration was set to 30 g/L, the maximum glucose concentration reached 17.66 g/L at 48 h after starch hydrolysis, and then, 2.36 g/L of acetic acid was obtained at 96 h. The final output of mcl-PHA was 0.5 g/L at 144 h, overall productivity for mcl-PHA was 3.47 mg/(L·h) and the total starch to mcl-PHA yield for the process was 16.67 mg/g. Although the overall yield and conversion rate of this process were not high, this is the first attempt to produce mcl-PHA using starch as a substrate, and it provides a feasible strategy for producing PHA from kitchen waste. The production process of mcl-PHA with a clear flora structure and short fermentation cycle was realized. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 2190572X
- Volume :
- 10
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- 3 Biotech
- Publication Type :
- Academic Journal
- Accession number :
- 144730775
- Full Text :
- https://doi.org/10.1007/s13205-020-02347-6