A novel process for the preparation of Cys-Si-NIPAM as a stationary phase of hydrophilic interaction liquid chromatography (HILIC).

l -Cysteine (L-Cys) and N-Isopropyl acrylamide (NIPAM)-modified silica spheres as a novel stationary phase for hydrophilic interaction liquid chromatography (HILIC) was produced firstly by cross-linking polymerization. Some characterizations in this article confirmed that the synthesis of Cys-Si-NIPAM is successful. Some polymer layers can be observed through transmission electron microscopy (TEM). In addition, through nitrogen adsorption porosity method, scanning electron microscopy (SEM), thermal gravimetric analysis (TGA) and other characterization methods, we can find the significant changes after modify. It has good hydrophilic property and higher column effect than bare silica column, Si-Cys column and Si-NIPAM column under the same conditions. It has good separation effect for some hydrophilic analytes such as 5 nucleosides and nucleoside bases, 5 amino acids, 4 sulfonamide drugs and 10 saccharides. The effects including column temperature, pH and organic solvent content on chromatographic performance were studied, which proved that hydrophilic interactions can be simultaneously existed between the stationary phase and the analytes. In addition, reproducibility and efficiency of the Cys-Si-NIPAM column was also investigated, the results illustrated that the stationary phase have passable stability (the intraday RSDs 0.08–0.44%, n = 3 and the interday RSD 0.46–3.50%, n = 3) and ideal efficiency (plates per meter, ~45700 plates/m). In conclusion, the preparation process of this hydrophilic liquid chromatography stationary phase is not only simple, but also can meet the basic requirements for the separation of hydrophilic analytes. Image 1 • We combine the process of the coating and polymerization into one step in the preparation of stationary phase. • The method of solidified gel-coated silica spheres for hydrophilic liquid chromatography stationary phase was first proposed. • The separation mechanism of the column depends on the l-Cysteine and N-Isopropyl acrylamide used for surface modification. • Surface modification of silica spheres by cross-linking polymerization and thiol olefin click reaction proved to be feasible. [ABSTRACT FROM AUTHOR]