Back to Search
Start Over
IL-34 regulates the inflammatory response and anti-bacterial immune defense of Japanese flounder Paralichthys olivaceus.
- Source :
-
Fish & Shellfish Immunology . Sep2020, Vol. 104, p228-236. 9p. - Publication Year :
- 2020
-
Abstract
- Interleukin (IL)-34 is a relatively recently discovered cytokine with pleiotropic effects on various cellular activities, including immune response. In fish, the knowledge on the function of IL-34 is limited. In the present work, we investigated the function of Japanese flounder Paralichthys olivaceus IL-34 (PoIL-34) in association with inflammation and immune defense. PoIL-34 possesses the conserved structure of IL-34 superfamily and shares 21.52% sequence identity with murine IL-34. PoIL-34 expression was detected in a wide range of tissues of flounder, in particular intestine, and was regulated to a significant extent by bacterial infection in a time-dependent fashion. In vitro studies showed that recombinant PoIL-34 (rPoIL-34) bound peripheral blood leukocytes (PBLs) and promoted ROS production, acid phosphatase activity, and cellular resistance against bacterial infection. At the molecular level, rPoIL-34 enhanced the expressions of inflammatory cytokines and specific JAK and STAT genes. Similar stimulatory effects of rPoIL-34 were observed in vivo. When PoIL-34 was overexpressed in flounder, the expressions of pro- and anti-inflammatory mediators were significantly affected in a tissue-dependent manner, which correlated with an augmented ability of the fish to eliminate invading pathogens from tissues. Together, these results indicated that PoIL-34 regulated inflammatory response probably via specific JAK/STAT pathways and had a significant influence on the immune defense of flounder against bacterial infection. • PoIL-34 expression occurred in multi-tissues and responded to bacterial infection. • rPoIL-34 bound to and promoted the activation and bacterial resistance of PBLs. • rPoIL-34 regulated the expressions of inflammatory cytokines and specific JAK/STAT. • In vivo PoIL-34 overexpession affected the expressions of inflammatory cytokines. • PoIL-34 overexpession reduced bacterial dissemination in fish tissues. [ABSTRACT FROM AUTHOR]
- Subjects :
- *INFLAMMATION
*PARALICHTHYS
*FLATFISHES
*ACID phosphatase
*IMMUNE response
Subjects
Details
- Language :
- English
- ISSN :
- 10504648
- Volume :
- 104
- Database :
- Academic Search Index
- Journal :
- Fish & Shellfish Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 145068355
- Full Text :
- https://doi.org/10.1016/j.fsi.2020.05.073