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Immunogenicity of SARS inactivated vaccine in BALB/c mice
- Source :
-
Immunology Letters . Sep2004, Vol. 95 Issue 2, p139-143. 5p. - Publication Year :
- 2004
-
Abstract
- Severe acute respiratory syndrome (SARS) is a serious infectious threat to public health. To create a novel trial vaccine and evaluate its potency, we attempted to generate a SARS inactivated vaccine using SARS coronavirus (SARS-CoV) strain F69 treated with formaldehyde and mixed with Al(OH)3. Three doses of the vaccine were used to challenge three groups of BALB/c mice. We found that the mice exhibited specific IgM on day 4 and IgG on day 8. The peak titers of IgG were at day 47 in low-dose group (1:19,200) and high-dose group (1:38,400) whereas in middle-dose group (1:19,200), the peak was at day 40. On day 63, the IgG levels reached a plateau. Neutralization assay demonstrated that the antisera could protect Vero-E6 cells from SARS-CoV''s infection. Analysis of the antibody specificity revealed that the mouse antisera contained a mixture of antibodies specifically against the structure proteins of SARS-CoV. Furthermore, the mouse antisera conferred higher amount of antibodies against protein N, polypeptide S4 and S2 than those of proteins M and 3CL. These findings suggest that the inactivated SARS-CoV could preserve its antigenicity and the inactivated vaccine can stimulate mice to produce high levels of antibodies with neutralization activity. Results also suggest that polypeptides originating from protein N or S might be a potential target for the generation of a recombinant SARS vaccine. [Copyright &y& Elsevier]
- Subjects :
- *SARS disease
*IMMUNOGLOBULINS
*CELLS
*PREVENTIVE medicine
Subjects
Details
- Language :
- English
- ISSN :
- 01652478
- Volume :
- 95
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Immunology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 14513043
- Full Text :
- https://doi.org/10.1016/j.imlet.2004.06.014