石斛灌胃对非酒精性脂肪肝小鼠肝细胞内脂肪过度沉积的改善作用及其机制.

Objective To observe the effect of dendrobium on the amelioration of excessive fat deposition in the liver cells of non-alcoholic fatty liver disease (NAFLD) mice, and to explore its mechanism. Methods Ten C57Bl/6J male mice were taken as the normal control group and fed with ordinary diet. The remaining 30 mice were fed with high-sugar and high-fat diet for 12 weeks. After the NAFLD models were established, they were randomly divided into the model group, low-dose and high-dose dendrobium groups, with 10 mice in each group. The mice in the low-dose and high-dose dendrobium groups were given 200 and 400 mg/( kg · d) dendrobium decoction by gavage, respectively. Both the mice in the model group and the normal group were given equal volume of normal saline for 14 weeks. After the treatment, the sta tus of the mice in each group, including body shape, weight, abdominal circumference, fur gloss, and activity level were observed. After the gavage, the mice in each group were fasted for 12 h, and blood was taken from the venous plexus under the eyes, and the serum TG, TC, AST and ALT were detected with a biochemical test kit. After the gavage, the mice were sacrificed, and the liver tissues were taken to observe the liver tissue pathology. PT-PCR was used to detect sterol regulatory element binding protein (SREBP-lc) mRNA and fatty acid synthase (FAS) mRNA in the liver tissues of mice in each group. Western blotting was used to detect the p-mTOR protein in the liver tissues of mice in each group. Results The body shape of the mice in the model group was significantly larger than that of the normal group, and their weight and ab dominal circumference also significantly increased. They moved freely, but their fur was rough and dull and easy to shed hair. The mice in the low-dose and high-dose dendrobium groups had normal activity, agile activity, normal spirit, and shiny fur. The serum TG, TC, AST and ALT levels of mice in the normal group, low-dose and high-dose dendrobium groups were lower than those in the model group (all P < 0. 05) . Mice in the model group had enlarged liver cells, more lipid droplets, and fatty cyst formation; the fatty liver transformation decreased in liver of the mice in the low-dose and high-dose dendrobium groups. The relative expression levels of SREBPlc mRNA and FAS mRNA in the liver tissues of mice in the normal group, low-dose and high-dose dendrobium groups were lower than those in the model group (all P <0. 05), and the relative expression ofSREBPlc mRNA and FAS mRNA of liver tissues in high-dose Dendrobium group was lower than that of the low-dose dendrobium group (all P < 0. 05). The relative expression of p-mTOR protein in the liver tissue of mice in the normal group, low-dose and high-dose dendrobium group was lower than that in the model group ( all P < 0. 05), and the relative expression level of p-mTOR protein in the liver tissues of the high-dose dendrobium group was low er than that of the low-dose dendrobium group ( P < 0. 05). Conclusion Dendrobium may regulate the mTOR signaling pathway to ameliorate the excessive fat deposition in the liver cells of NAFLD mice. [ABSTRACT FROM AUTHOR]