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LncRNA-599554 sponges miR-15a-5p to contribute inductive ability of dermal papilla cells through positive regulation of the expression of Wnt3a in cashmere goat.
- Source :
-
Electronic Journal of Biotechnology . May2020, Vol. 45, p19-29. 11p. - Publication Year :
- 2020
-
Abstract
- Background: Long non-coding RNAs (lncRNAs), as post-transcriptional regulators, were thought to function in the inductive property of dermal papilla cells (DPCs) in cashmere goat. Previously, lncRNA-599554 was identified in secondary hair follicle (SHF) of cashmere goat, but its functional significance is unknown. Results: In the present investigation, we verified that lncRNA-599554 had significantly higher expression at the anagen dermal papilla of cashmere goat SHF than that at telogen. Based on overexpression and knockdown techniques, we found that lncRNA-599554 contributes the inductive property of DPCs of cashmere goat, which was assessed by detecting the changes in the expression of several typical indictor genes in DPCs including ET- 1, SCF, Versican, ALP, Lef1 and Ptc-1. Based on RNA pull-down assay, we verified that lncRNA-599554 directly interacted with chi-miR-15a-5p. Also, we showed that lncRNA-599554 positively regulated the Wnt3a expression in DPCs but which did not appear to involve its modulating of promoter methylation. Based on the use of Dual-luciferase reporter assays, our data indicated that lncRNA-599554 regulated the Wnt3a expression through chi-miR-15a-5p-mediated post-transcriptional level. Conclusions:Weshowed that lncRNA-599554 contributes the inductive property of DPCs in cashmere goatwhich might be achieved through sponging chi-miR-15b-5p to promote the Wnt3a expression. The results from the present investigation provided a novel insight into the functional mechanism of lncRNA-599554 in the SHF regeneration of cashmere goat along with the formation and growth of cashmere fiber. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CASHMERE
*GOATS
*HAIR follicles
*NON-coding RNA
*CELLS
Subjects
Details
- Language :
- English
- ISSN :
- 07173458
- Volume :
- 45
- Database :
- Academic Search Index
- Journal :
- Electronic Journal of Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 145333537
- Full Text :
- https://doi.org/10.1016/j.ejbt.2020.03.002