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Acquisition of Plasmid-Mediated Colistin Resistance Gene mcr-1 in Escherichia coli of Livestock Origin in Bangladesh.
- Source :
-
Microbial Drug Resistance: Mechanism, Epidemiology, & Disease . Sep2020, Vol. 26 Issue 9, p1058-1062. 5p. - Publication Year :
- 2020
-
Abstract
- Aims: To investigate plasmid-borne colistin resistance mechanism (plasmid-mediated colistin resistance [mcr-1]) in Escherichia coli of human, veterinary, and environmental origin in Bangladesh. Materials and methods: A total of 810 samples were collected from different sources. Isolation and identification of E. coli was performed using classical bacteriology and then tested for antimicrobial susceptibility. Colistin-resistant isolates were further analyzed for mcr-1 gene using PCR. Minimum inhibitory concentration (MIC) was determined using microbroth dilution technique. After sequencing of mcr-1 gene, phylogenetics was conducted to see the relationship with other mcr-1 gene sequences. Results: A total of 358 E. coli were isolated from 810 samples of humans, animals, environment, and food in Bangladesh. Of them 49 (15.9%) isolates were phenotypically resistant to colistin and 254 (70.9%) were resistant to multiple antimicrobials. mcr-1 gene was detected in three E. coli isolates of poultry source. For the three mcr-1 positive isolates the MIC of colistin sulfate was 4, 8, and 128 μg/mL. Gene sequencing of two of the three mcr-1 positive isolates and phylogenetic analysis showed close similarities of one isolate to other mcr-1 sequences available at GenBank while the other appeared to have evolved locally. Conclusion: First-ever report on circulation of mcr-1 E. coli of livestock origin in Bangladesh. [ABSTRACT FROM AUTHOR]
- Subjects :
- *COLISTIN
*ESCHERICHIA coli
*MICROBIAL sensitivity tests
*LIVESTOCK
*GENES
Subjects
Details
- Language :
- English
- ISSN :
- 10766294
- Volume :
- 26
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Microbial Drug Resistance: Mechanism, Epidemiology, & Disease
- Publication Type :
- Academic Journal
- Accession number :
- 145701112
- Full Text :
- https://doi.org/10.1089/mdr.2019.0304