STUDIES ON EOSINOPHIL LEUCOCYTE MIGRATION I. EOSINOPHIL AND NEUTROPHIL ACCUMULATION FOLLOWING ANTIGEN-ANTIBODY REACTIONS IN GUINEA-PIG SKIN.

Eosinophil leucocytes migrated into the site of PCA reactions mediated by those fractions of 7S guinea-pig lgG containing lgG1; neutrophils were associated with IgG1 and IgG2. Maximal eosinophil infiltration is seen at 12 hr and was associated with degranulation. Intradermal histamine was not eosinophilotactic in guinea-pigs. Performed antigen-antibody complexes of IgG1 and IgG2 both promoted eosinophils and neutrophil migration in guinea-pig skin but slightly more eosinophils were seen following injections of complexes containing IgG1. Local eosinophilia and PCA activity were mediated by a relatively heat-stable element since these effects were demonstrable even after prolonged heating of fractions containing IgG1. Eosinophils were seen following injections of Compound 48/80 and this was accompanied by low mast cell counts; however, there was also some associated tissue destruction. [ABSTRACT FROM AUTHOR]