Back to Search
Start Over
Phospholipase C-γ1 is a guanine nucleotide exchange factor for dynamin-1 and enhances dynamin-1-dependent epidermal growth factor receptor endocytosis.
- Source :
-
Journal of Cell Science . 8/1/2004, Vol. 117 Issue 17, p3785-3795. 11p. - Publication Year :
- 2004
-
Abstract
- Phospholipase C-γ1 (PLC-γ1), which interacts with a variety of signaling molecules through its two Src homology (SH) 2 domains and a single SH3 domain has been implicated in the regulation of many cellular functions. We demonstrate that PLC-γ1 acts as a guanine nucleotide exchange factor (GEF) of dynamin-1, a 100 kDa GTPase protein, which is involved in clathrin-mediated endocytosis of epidermal growth factor (EGF) receptor. Overexpression of PLC-γ1 increases endocytosis of the EGF receptor by increasing guanine nucleotide exchange activity of dynamin-1. The GEF activity of PLC-γ1 is mediated by the direct interaction of its SH3 domain with dynamin-1. EGF- dependent activation of ERK and serum response element (SRE) are both up-regulated in PC12 cells stably overexpressing PLC-γ1, but knockdown of PLC-γ1 by siRNA significantly reduces ERK activation. These results establish a new role for PLC-γ1 in the regulation of endocytosis and suggest that endocytosis of activated EGF receptors may mediate PLC-γl-dependent proliferation. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PHOSPHOLIPASES
*MOLECULES
*HOMOLOGY (Biology)
*CELLS
*ENDOCYTOSIS
*G proteins
Subjects
Details
- Language :
- English
- ISSN :
- 00219533
- Volume :
- 117
- Issue :
- 17
- Database :
- Academic Search Index
- Journal :
- Journal of Cell Science
- Publication Type :
- Academic Journal
- Accession number :
- 14584012
- Full Text :
- https://doi.org/10.1242/jcs.01220