Detection of Glyamifop residues in rice and its environment by the QuEChERS method combined with HPLC–MS.

• In this study, the QuEChERS and HPLC–MS methods were employed for residue analysis of Glyamifop in rice and its environment. • Quantification done by HPLC–MS (Ion trap). • It provides a reference for the detection Glyamifop herbicide in natural environment. Glyamifop is a new herbicide developed by Jiangsu Zhongqi Technology Co., Ltd. (Nanjing, China). No analytical method has previously been reported for determining its residual concentrations in rice and its related environmental samples. This work established a method for the determination of Glyamifop residues in various matrices using high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS) with sample pretreatment by the optimized quick, easy, cheap, effective, rugged, and safe (QuEChERS) method. All samples were extracted with acetonitrile and purified with octadecyl bonded silica gel, N -propylethylenediamine, and graphitized carbon black adsorbents. Chromatographic separation was performed on an Agilen ZORBAX Eclipse XDB-C18 column, and Glyamifop in the samples was detected by electrospray ionization mass spectrometry in positive ion mode. Results demonstrated this method has good linearity for Glyamifop assessment in all matrices over a wide concentration range (0.01–1 mg L−1), with a coefficient of determination >0.99. At levels of 0.01, 0.1, and 0.5 mg kg−1, the mean recoveries of Glyamifop in various matrices varied between 84.15% and 105.76%, with relative standard deviations of 1.3%–9.8%. The detection and quantification limits of this method in each matrix are 1.3–3.6 μg kg−1 and 4.89–12.48 μg kg−1, respectively. All matrices had a moderate matrix effect (between −21.63% and 14.21%), which could be calibrated using respective matrix standard curves. The proposed method is simple and sensitive, and appears suitable for the determination of Glyamifop residues in rice and its environment. [ABSTRACT FROM AUTHOR]