Back to Search
Start Over
A water-soluble probe with p-hydroxybenzyl quaternary ammonium linker for selective imaging in senescent cells.
- Source :
-
Analytica Chimica Acta . Oct2020, Vol. 1133, p99-108. 10p. - Publication Year :
- 2020
-
Abstract
- A water-soluble probe with p-hydroxybenzyl quaternary ammonium linker, FR-2a, for selective imaging in senescent cells is reported. Probe FR-2a integrated water-soluble fluorophore (HT-4a) and β-galactosidase (β-gal) trigger into one entity by a p-hydroxybenzyl quaternary ammonium linker. HT-4a is a styryl-based push–pull benzothiazole fluorophore with attractive properties, including excellent water-solubility, intense fluorescence emission and a large Stokes shift (161 nm), characterized by an intramolecular charge transfer (ICT) excited state. The formation of quaternary ammonium deactivated the ICT state, resulting in fluorescence quenching of FR-2a. In the presence of β-gal, the glycosidic bond was hydrolyzed and fluorophore HT-4a was released through self-immolative process, resulting in effective fluorescence recovery. FR-2a shows high affinity to β-gal (K m = 1.33 μM), exhibiting good sensitivity, selectivity and stability for imaging in senescent cells. Image 1 • Fluorescent probe FR-2a with self-immolative p-hydroxybenzyl quaternary ammonium linker was developed. • FR-2a selectively and rapidly responses to β-gal in PBS buffer. • FR-2a has excellent water solubility, avoiding ACQ of fluorescence in PBS. • FR-2a was capable of selective imaging in senescent cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032670
- Volume :
- 1133
- Database :
- Academic Search Index
- Journal :
- Analytica Chimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 146100060
- Full Text :
- https://doi.org/10.1016/j.aca.2020.07.079