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The Beginning of the End: A Chromosomal Assembly of the New World Malaria Mosquito Ends with a Novel Telomere.
- Source :
-
G3: Genes | Genomes | Genetics . Oct2020, Vol. 10 Issue 10, p3811-3819. 9p. - Publication Year :
- 2020
-
Abstract
- Chromosome level assemblies are accumulating in various taxonomic groups including mosquitoes. However, even in the few reference-quality mosquito assemblies, a significant portion of the heterochromatic regions including telomeres remain unresolved. Here we produce a de novo assembly of the New World malaria mosquito, Anopheles albimanus by integrating Oxford Nanopore sequencing, Illumina, Hi-C and optical mapping. This 172.6 Mbps female assembly, which we call AalbS3, is obtained by scaffolding polished large contigs (contig N50 = 13.7 Mbps) into three chromosomes. All chromosome arms end with telomeric repeats, which is the first in mosquito assemblies and represents a significant step toward the completion of a genome assembly. These telomeres consist of tandem repeats of a novel 30-32 bp Telomeric Repeat Unit (TRU) and are confirmed by analyzing the termini of long reads and through both chromosomal in situ hybridization and a Bal31 sensitivity assay. The AalbS3 assembly included previously uncharacterized centromeric and rDNA clusters and more than doubled the content of transposable elements and other repetitive sequences. This telomere-to-telomere assembly, although still containing gaps, represents a significant step toward resolving biologically important but previously hidden genomic components. The comparison of different scaffolding methods will also inform future efforts to obtain reference-quality genomes for other mosquito species. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MOSQUITOES
*MALARIA
*TANDEM repeats
*IN situ hybridization
*ANOPHELES
*CENTROMERE
Subjects
Details
- Language :
- English
- ISSN :
- 21601836
- Volume :
- 10
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- G3: Genes | Genomes | Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 146350444
- Full Text :
- https://doi.org/10.1534/g3.120.401654