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Soybean nuclear factor YA10 positively regulates drought resistance in transgenic Arabidopsis thaliana.
- Source :
-
Environmental & Experimental Botany . Dec2020, Vol. 180, pN.PAG-N.PAG. 1p. - Publication Year :
- 2020
-
Abstract
- • The GmNF-YA10 gene encodes an NF-YA transcription factor located in the nucleus that responds to PEG and ABA treatment at the transcription level. • Overexpression of the GmNF-YA10 gene increases the drought resistance of transgenic Arabidopsis by accumulating osmotic substances, slowing membrane lipid peroxidation, enhancing photosynthesis, regulating ABA-mediated stomatal closure, reducing the leaf water loss rate, and affecting the transcript levels of stress-related genes. • GmNF-YA10 gene participates in Arabidopsis response to drought stress through two pathways, ABA-dependent and ABA-independent. Nuclear factor (NF)-Y is composed of three subunits, NF-YA, NF-YB and NF-YC, and plays important roles in the regulation of plant abiotic stress response gene expression. However, the function of individual soybean NF-YA proteins in response to drought stress is unclear. In this study, the soybean NF-YA subunit gene GmNF-YA10 was cloned, and its function in response to drought stress was identified. GmNF-YA10 is broadly expressed in soybean tissues and is specifically located in the nucleus. In soybean roots, the transcription level of the GmNF-YA10 gene was induced by polyethylene glycol (PEG)-simulated drought stress and abscisic acid (ABA) treatment. Under mannitol stress, the germination rate of transgenic Arabidopsis overexpressing GmNF-YA10 was higher than that of wild-type (WT) Arabidopsis. After drought stress, the survival rate, relative water content, ABA content, proline content and chlorophyll content of transgenic Arabidopsis overexpressing GmNF-YA10 were higher than those of WT Arabidopsis while the rate of water loss and the contents of malondialdehyde and sucrose were lower than those of WT Arabidopsis , indicating that transgenic Arabidopsis overexpressing GmNF-YA10 has better drought resistance than WT Arabidopsis. Under the ABA treatment, the root length of transgenic Arabidopsis overexpressing GmNF-YA10 was shorter than that of WT Arabidopsis and the degree of stomatal closure was greater than that of WT Arabidopsis , indicating that transgenic Arabidopsis overexpressing GmNF-YA10 was more sensitive to ABA at the post-germination stage. Transcriptome sequencing and real-time quantitative PCR analysis showed that GmNF-YA10 affected the expression of both ABA-dependent and ABA-independent signalling pathway genes. These results indicate that GmNF-YA10 significantly improves the drought resistance of transgenic Arabidopsis through ABA-dependent and ABA-independent signal transduction pathways. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00988472
- Volume :
- 180
- Database :
- Academic Search Index
- Journal :
- Environmental & Experimental Botany
- Publication Type :
- Academic Journal
- Accession number :
- 146413365
- Full Text :
- https://doi.org/10.1016/j.envexpbot.2020.104249