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Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK.
- Source :
-
Biotechnology for Biofuels . 10/19/2020, Vol. 13 Issue 1, p1-14. 14p. - Publication Year :
- 2020
-
Abstract
- Background: To meet the present transportation demands and solve food versus fuel issue, microbial lipid-derived biofuels are gaining attention worldwide. This study is focussed on high-throughput screening of oleaginous yeast by microwave-aided Nile red spectrofluorimetry and exploring pongamia shell hydrolysate (PSH) as a feedstock for lipid production using novel oleaginous yeast Rhodotorula pacifica INDKK. Results: A new oleaginous yeast R. pacifica INDKK was identified and selected for microbial lipid production. R. pacifica INDKK produced maximum 12.8 ± 0.66 g/L of dry cell weight and 6.78 ± 0.4 g/L of lipid titre after 120 h of growth, showed high tolerance to pre-treatment-derived inhibitors such as 5-hydroxymethyl furfural (5-HMF), (2 g/L), furfural (0.5 g/L) and acetic acid (0.5 g/L), and ability to assimilate C3, C5 and C6 sugars. Interestingly, R. pacifica INDKK showed higher lipid accumulation when grown in alkali-treated saccharified PSH (AS-PSH) (0.058 ± 0.006 g/L/h) as compared to acid-treated detoxified PSH (AD-PSH) (0.037 ± 0.006 g/L/h) and YNB medium (0.055 ± 0.003 g/L/h). The major fatty acid constituents are oleic, palmitic, linoleic and linolenic acids with an estimated cetane number (CN) of about 56.7, indicating the good quality of fuel. Conclusion: These results suggested that PSH and R. pacifica INDKK could be considered as potential feedstock for sustainable biodiesel production. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17546834
- Volume :
- 13
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Biotechnology for Biofuels
- Publication Type :
- Academic Journal
- Accession number :
- 146531363
- Full Text :
- https://doi.org/10.1186/s13068-020-01814-9