PCR-based specific detection ofRalstonia solanacearumrace 4 strains.

Two primer sets were designed based on the sequence of polymorphic bands that were derived from repetitive sequence-based polymerase chain reaction (rep-PCR) fingerprinting and specifically detected inRalstonia solanacearumrace 4 strains (ginger, mioga, and curcuma isolates). One primer set (AKIF-AKIR) amplified a single band (165?bp) from genomic DNA obtained from all mioga and curcuma and some ginger isolates; another set (21F-21R) amplified one band (125?bp) from the other ginger isolates. These primer sets did not amplify the bands from genomic DNA of otherR. solanacearumstrains or of other related bacteria. PCR detection limit for the pathogen was 2 × 102?cfu. [ABSTRACT FROM AUTHOR]