Sensitive biosensor for chronic myeloid leukemia detection using multi-wall carbon nanotube.

This work presents the fabrication of a resistive biosensor to detect chronic myeloid leukemia by targeting leukemia biomarker that is P-glycoprotein attached to surface of the cell. The sensor was fabricated using multi-wall carbon nanotubes due to its high surface area, and optimized parameters such as silane binding time and antibody content were done for its efficient operation. The electrode was characterized using SEM, Raman and FTIR studies. Further, sensing was done using leukemia K562 cell line in a linear concentration range of 2x106 cells/mL – 2x103 cells/mL. The calibration plot (change in resistance v/s log concentration of K562 cells) demonstrated linearity having the regression coefficient of 0.8518 and lower detection threshold was found to be 11 cells/mL. The observed sensitivity is close to the reports available in literature where additional rigorous steps are required for fabrication. The sensing phenomenon can be simulated with Fermi level fluctuation when cell binds/ detaches on/ from the electrode surface. The sensor was found to be sensitive, cost effective, less reagents requirement and quick than the popular technologies used in hospitals. [ABSTRACT FROM AUTHOR]