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Antimicrobial peptides-loaded smart chitosan hydrogel: Release behavior and antibacterial potential against antibiotic resistant clinical isolates.
- Source :
-
International Journal of Biological Macromolecules . Dec2020, Vol. 164, p855-862. 8p. - Publication Year :
- 2020
-
Abstract
- In this study, we synthesized thermo-responsive chitosan (TCTS) hydrogels, and loaded with different concentrations of antimicrobial peptide (AMP) (0, 4, 8 and 16 μg·ml−1) to fabricate an antibacterial wound dressing against resistant clinical isolates. Physico-chemical properties, release behavior, cytobiocompatibility and antibacterial activity of the AMP-TCTS hydrogels against standard strain and resistant Acinetobacter baumannii were fully determined in vitro. The TCTS-40% β-glycerolphosphate hydrogels showed a gelation time of 15 min at 37 °C. 80% weight loss at day 35 with no changes in pH value was observed. AMP-TCTS hydrogels showed a burst release of AMP (around 40%) at day 1, and a controlled release up to day 7. A dramatic water uptake was observed at first 4 h, and then continued for 10 h in a steady manner. All the AMP-TCTS hydrogels showed excellent cytobiocompatibility for human fibroblasts. The TCTS showed no antibacterial activity against both standard strain and clinical isolates. All the AMP-TCTS hydrogels had strong antibacterial activity against standard strains, but only 16 μg·ml−1 showed antibacterial behavior against resistant A. baumannii. Our results strongly suggest the 16 μg·ml−1 AMP-TCTS hydrogel as an excellent antibacterial wound dressing against resistant A. baumannii , and now promises to proceed with pre-clinical investigations. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANTIMICROBIAL peptides
*HYDROGELS
*ACINETOBACTER baumannii
*GELATION
*CHITOSAN
Subjects
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 164
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 146831408
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2020.07.011