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Identification of meiotic anomalies with multiplex fluorescence in situ hybridization: Preliminary results
- Source :
-
Fertility & Sterility . Sep2004, Vol. 82 Issue 3, p712-717. 6p. - Publication Year :
- 2004
-
Abstract
- To characterize meiotic anomalies in infertile men by multiplex fluorescence in situ hybridization (M-FISH) and to determine whether synaptic problems affect specific bivalents or whether anomalies are random.Analysis of meiotic preparations with standard techniques and M-FISH.Assisted reproduction centers and Universitat Autònoma de Barcelona.Three fertile men undergoing vasectomy, four sterile patients with oligoasthenoteratozoospermia, and one patient with a Robertsonian translocation t(13;14).Unilateral testicular biopsy in controls and patients with oligoasthenoteratozoospermia and collection of a semen sample from the translocation carrier.Identification of bivalents in metaphase I and chromosomes in metaphase II and characterization of chromosome abnormalities.All bivalents in metaphase I and all chromosomes in metaphase II could be identified. In controls and in one patient with oligoasthenoteratozoospermia, meiosis was normal. Other patients with oligoasthenoteratozoospermia showed different types of anomaly: desynapsis, breaks, precocious XY separation, or cryptic reorganizations. The Robertsonian translocation t(13;14) was easily identified.Results confirm the high incidence of synaptic errors in oligoasthenoteratozoospermia patients. Bivalents in metaphase I and chromosomes in metaphase II were individually identifiable. Nondisjunctional errors or small reorganizations overlooked in classic meiotic preparations were identified. Synaptic anomalies seem to affect meiotic bivalents at random. [Copyright &y& Elsevier]
- Subjects :
- *GENETICS
*KARYOKINESIS
*CYTOTAXONOMY
*FALLIBILITY
Subjects
Details
- Language :
- English
- ISSN :
- 00150282
- Volume :
- 82
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Fertility & Sterility
- Publication Type :
- Academic Journal
- Accession number :
- 14685840
- Full Text :
- https://doi.org/10.1016/j.fertnstert.2004.02.122