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TEAD4 promotes tumor development in patients with lung adenocarcinoma via ERK signaling pathway.

Authors :
Gu, Chang
Huang, Zhenyu
Chen, Xiaojian
Liu, Chenying
Rocco, Gaetano
Zhao, Shengnan
Xie, Huikang
Chen, Jiafei
Dai, Chenyang
Chen, Chang
Source :
BBA: Molecular Basis of Disease. Dec2020, Vol. 1866 Issue 12, pN.PAG-N.PAG. 1p.
Publication Year :
2020

Abstract

Whether TEAD4 itself plays a vital role in the tumorigenesis and development of lung adenocarcinoma remains unclear. In our study, we aim to investigate the expression pattern and biological functions of TEAD4 and further investigate the potential mechanisms. Clinical tumor and paired normal samples were collected for preparing tissue microarray. Western blot and immunohistochemical (IHC) staining of TEAD4 expression in these tissues were conducted to explore the expression pattern. Moreover, A549 cell line was select for investigating the function of TEAD4 for lung adenocarcinoma in vitro and in vivo. RNA sequencing was finally performed to further detect the potential downstream genes. The elevated TEAD4 expression level was observed in tumor tissues and the patients with higher TEAD4 expression tended to have worse overall survival. The knockdown of TEAD4 inhibits A549 cells proliferation ability and migration ability. A total of 431 differentially expressed genes (DEGs), including 239 down-regulated genes and 191 up-regulated genes, were finally identified and some of DEGs were validated. Moreover, knockdown of TEAD4 led to the down-regulation of pERK, which maybe the potential TEAD4-targeted signaling pathway to play the pro-tumorigenic function. The expression level of TEAD4 is high in lung adenocarcinoma tumor tissues and positively associated with worse prognosis. Up-regulation of TEAD4 may lead to excessive transcription and phosphorylation of ERK proteins and therefore accelerates the process of tumor development. Our results demonstrate that overexpression of TEAD4 is a new mechanism of dysregulation of Hippo pathway. • TEAD4 promotes tumor development in patients with lung adenocarcinoma via ERK signaling pathway. • Overexpression of TEAD4 is a new mechanism of dysregulation of Hippo pathway. • The expression level of TEAD4 is high in tumor tissues and positively associated with worse prognosis. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09254439
Volume :
1866
Issue :
12
Database :
Academic Search Index
Journal :
BBA: Molecular Basis of Disease
Publication Type :
Academic Journal
Accession number :
147017835
Full Text :
https://doi.org/10.1016/j.bbadis.2020.165921