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Characteristics of HIV-1 env genes from Chinese chronically infected donors with highly broad cross-neutralizing activity.
- Source :
-
Virology . Dec2020, Vol. 551, p16-25. 10p. - Publication Year :
- 2020
-
Abstract
- Knowledge about the special characteristics of HIV-1 envelope (env) glycoproteins in rare individuals developing >90% neutralization breadth in Chinese subtype B' slow progressors may provide insights for vaccine design against local viruses. We performed a cross-sectional analysis on 7 samples. We tested the neutralization breadth and geometric mean ID50 titers (GMTs) of these samples, and divided them into hBCN+ and hBCN- group according to whether their neutralization breadth >90%. We obtained env sequences in these samples through single genome amplification (SGA) assay. By comparing with hBCN-, subtype B chronically infected group (B-SP), and Chinese subtype B group (B-Database), we analyzed the characteristics of the env sequences of hBCN+ group. Longer V1 and V4 regions with more glycosylation sites were found in hBCN+ samples compared to hBCN- samples. Further analysis compared to B-SP and B-Database showed that hBCN+ group exhibited unique extra-long V1 region containing higher proportion of N-glycan sites and additional cysteines. [ABSTRACT FROM AUTHOR]
- Subjects :
- *NEUTRALIZATION tests
*GENES
*CROSS-sectional method
*GLYCOSYLATION
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Volume :
- 551
- Database :
- Academic Search Index
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 147117433
- Full Text :
- https://doi.org/10.1016/j.virol.2020.08.012