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Effect of Vitis vinifera L. cv. Chardonnay xylem fluid on cecropin B activity against Xylella fastidiosa
- Source :
-
Physiological & Molecular Plant Pathology . Feb2004, Vol. 64 Issue 2, p73-81. 9p. - Publication Year :
- 2004
-
Abstract
- Cecropin B (CB) is a very efficient antimicrobial agent against Xylella fastidiosa. CB activity decreased after incubation with xylem fluid from Vitis vinifera cv. Chardonnay. SDS-PAGE demonstrated the existence of protein(s) in xylem fluid of V. vinifera cv. Chardonnay that interact with CB and inhibit CB activity. The antimicrobial activity of CB was dependent on incubation time and colony size of X. fastidiosa. X. fastidiosa colonies in periwinkle wilt medium plus (PW+), and chemically defined media (3G10-R and CHARD2) showed different patterns in colony size and colony number in these media. After 1h of incubation in 10μM CB, only large-sized colonies were observed in the three media. These results suggested that most of the bacterial cells or small aggregates in suspension were killed by CB. Aggregation of X. fastidiosa cells may serve as a mechanism of protection against CB. [Copyright &y& Elsevier]
- Subjects :
- *PLANT cells & tissues
*VASCULAR system of plants
*ANTI-infective agents
*CECROPIA
Subjects
Details
- Language :
- English
- ISSN :
- 08855765
- Volume :
- 64
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Physiological & Molecular Plant Pathology
- Publication Type :
- Academic Journal
- Accession number :
- 14714803
- Full Text :
- https://doi.org/10.1016/j.pmpp.2004.07.001